![]() ![]() Then, the numerical densities and total numbers of ovarian granulosa cells (OGCs) and primordial, preantral and antral follicles in serial sections were estimated using design-based stereology. The volume and volume fractions of ovaries were measured by the Cavalieri method. One of the two paraffin-embedded ovaries of each mouse was cut into 5 µm sections, and the other was cut into 15 µm sections and then subjected to haematoxylin and eosin staining and anti-follicle stimulating hormone receptor antibody immunohistochemistry. In this study, six female mice were used to acquire the ovarian tissues. Stereology is an accurate method to obtain quantitative 3D information and the stereological data is the basis for 3D bioprinting ovaries. The use of 3D-bioprinted ovaries has been proven to be a promising technique for preserving fertility. ![]()
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